These instructions accompany a downloadable software bundle posted at

http://dispimlineage.wormguides.org

Proper installation of the bundled programs and files onto a 64-bit Windows PC with a CUDA-enabled GPU card is required to follow the data processing protocol described in Duncan et al., JoVE 2019.  These steps take only a few minutes.

1. The bundle can be obtained directly from this link
	downloadBundleFordiSPIMLineaging_ExtractIntoCdriveRootDirectory.zip

2.  Use right-click>Extract All… to extract the zipped contents to the top-level folder C:\ 

3.  Navigate to the newly unpacked folder C:\CytoSHOWextrasForC\SNFeeder_compiled\packaged\ and double-click on the program SN_Feeder_Installer.exe

4. Accept license agreements and the series of prompts to install detect_track_driver_allmatlab.exe and the MATLAB Compiler Runtime at the default locations.  Both are free of charge and do not require the installation of MATLAB itself.

5.  Copy these two files to your Windows Desktop for easy access:
	C:\CytoSHOWextrasForC\CytoSHOW_APP.jnlp
	C:\CytoSHOWextrasForC\AceTree_16bitCompat.jar

6. Ensure that your PC has the proper version of Java installed.  The 64-bit Windows Java installer is at 
	https://javadl.oracle.com/webapps/download/AutoDL?BundleId=236888_42970487e3af4f5aa5bca3f542482c60

7.   Once steps 1-6 are completed, use the link
	http://run.cytoshow.org
or the Desktop icon
	CytoSHOW_APP.jnlp
to launch CytoSHOW with the fully enabled diSPIM lineaging data pipeline enabled.